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| Silva, S. R.; Cunha Neto, R. S.; Chaves, L. B.; Faquim-Mauro, E. L.; Silva, A. C. R.. |
| The currently recommended by WHO for prevention individuals exposed to rabies virus (RV) is pre-exposure prophylaxis (PEP) through the administration of three doses of rabies vaccine by intradermal (ID) or intramuscular (IM) route. The previous study showed that the persistence of protective antibodies titer in the IM schedule is more long-lived, suggesting a different clearance mechanism of antibody. It known that carbohydrate modification of antibodies could induce accelerated clearance or modulate of antibody effector functions. All endogenous IgG antibodies contain an N-linked glycan in the Fc region and about 20% contain glycans elsewhere on the molecule to maintain the two heavy chains of the Fc in an open conformation required for interactions with... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3101 |
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| Castilho, J. G.; Rodrigues, A. C.; Carnieli, P. J. R.; Oliveira, R. N.; Silva, A. C. R.; Caporale, G. M. M.; Brandão, P. E.; Batista, H. B. C. R.. |
| This study describes the comparison of in vivo and in vitro transfection of antibodies to use as a new mechanism for antiviral therapy against rabies. The transfection was made in vitro with neuroblastoma murine cell line (Neuro‐2a) and in vivo with 21 years old mice. Both cells and mice were infected with different infectious doses (0.1; 1.0; 10 and 100 ID50) of the rabies virus. After 24 hours the infected cells and mice were transfected with polyclonal antibodies against rabies produced in equines (unpurified hyperimmune serum ‐ total antibody) using a cationic reagent, the lipofectamine 2000 previously diluted 1:20, for the negative transfection control, only minimum essential medium was used. The ability of the antibodies to neutralize the rabies... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17374 |
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| Freitas, A. P.; Silva, A. C. R.; Scheffer, K. C.; Pimenta, D. C.. |
| Rabies is an infectious disease caused by a virus belonging to the family Lissavirus that affects all mammals including humans. Rabies transmission occurs by virus-containing saliva , through biting or licking of an injured tissue. The virus replicates at the site of inoculation until it reaches the nerve endings. At the neuromuscular junctions, the virus binds specifically to the nicotinic acetylcholine receptor. On the other hand, alkaloids have yielded interesting results in terms of drug development. Since rabies virus infection is, at least, partially associated to the nicotinic acetylcholine receptor, the present study focus on the evaluation of molecules obtained from the skin secretion of Ameerega picta, in the process of penetration and infection... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2570 |
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| Castilho, J. G.; Batista, H. B. C. R.; Rodrigues, A. C.; Carnieli Jr, P.; Oliveira, R. N.; Silva, A. C. R.; Caporale, G. M. M.; Carrieri, M. L.; Kotait, I.; Brandão, P. E.. |
| The aim of this study was to develop a new mechanism for antiviral therapy against rabies based on the introduction by transfection with a cationic reagent (lipofectamine 2000) of antibodies into neuronal cells infected with the rabies virus. N2A cells were infected using 96-well plates and different viral concentrations (0.1, 1.0, 10 and 100 TCID50) of three lineages of rabies virus circulating in Brazil (dog, Desmodus rotundus and Eptesicus furinalis). After incubation for 24 h, the cells were transfected with antirabies- virus polyclonal antibodies and lipofectamine 2000. These cells made up the treatment group (TG). The cells in the negative control group (CG) were treated with only Minimum Essential Medium. After 11 hours, the plates were fixed with... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3080 |
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| Scheffer, K. C.; Di Gregorio, M. C.; Batista, A. M.; Rosato, P. N.; Campos Filho, E.; Silva, A. C. R.; Caporale, G. M. M.; Cruz, P. S.; Chaves, L. B.. |
| Among the factors that establish the pre‐analytical variability, some studies report that hemolysis is the main interferential in several tests. However, no studies were found on immunofluorescence tests or that using cell cultures. The aim of this study was to evaluate the interference of different degrees of hemolysis serum samples in the evaluation of virus neutralizing antibodies (VNA) by rapid fluorescent focus inhibition test (RFFIT). We obtained 27 blood samples from dogs vaccinated for rabies. Hemolysis was caused by mechanical induction and freezing. Hemolysated sera were classified visually and by measurement of hemoglobin made by spectrophotometry. The evaluation of VNA in the samples was performed by RFFIT. The results were statistically... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/17379 |
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| Ferreira, J. S.; Trotti, A. C. P.; Scheffer, K. C.; Caporale, G. M. M.; Silva, A. C. R.; Chaves, L. B.. |
| The Fluorescent Antibody Virus Neutralization Test (FAVN) is used routinely in many laboratories for the reference measurement of rabies virus neutralizing antibodies (VNA) in serum of animals to confirm the efficacy of vaccination against rabies, which is required to authorize the transit of these animals in countries free of rabies. The World Organization for Animal Health (OIE) recommends a VNA title ≥ 0,5 IU/mL to ensure that the animal has immunity against the rabies virus. The rabies laboratory diagnosis of Pasteur Institute of São Paulo/Brazil (IP/SP) is a national reference of the Ministry of Health for rabies diagnosis and performs the measurement of VNA in humans and animals serum samples for proof of immunization against rabies. The objective of... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/2526 |
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| Silva, V. A.; Gamom, T. H. M.; Silva, A. C. R.; Caporale, G. M. M.; Chaves, L. B.; Scheffer, K. C.. |
| O principal objetivo da vacinação do Programa Nacional de Profilaxia da Raiva é manter índices imunogênicos protetores nos animais vacinados, esperando-se que os títulos de anticorpos neutralizantes (AcN) sejam ≥ 0,5UI/mL. Tanto a Organização Mundial da Saúde (OMS) como a Oficina Internacional de Epizootias (OIE) consideram essa titulação como referência de status de proteção contra o vírus da raiva e um indicador para avaliar a eficácia da vacina. O objetivo deste estudo foi avaliar, de acordo com a idade, raça e o período entre a aplicação da vacina e a colheita do sangue, a resposta imunológica de cães primo vacinados com vacina de cultivo celular. Para avaliar a resposta imune em cães foram analisadas 432 amostras recebidas no Instituto Pasteur de São... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/16813 |
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| Freitas, A. P.; Peixoto, Z. M. P.; Silva, A. C. R.; Scheffer, K. C.; Chaves, L. B.; Caporale, G. M. M.. |
| The fluorescent anti-rabies conjugate (FARC) produced in Instituto Pasteur de São Paulo (IP-SP) is obtained by fluorescein isothiocyanate (FITC) conjugation with specific polyclonal antibodies purified and it recommended storage temperature of 4°C protected from light incidence. Given the fundamental importance of this reagent for rabies laboratorial diagnosis, the aim of this study was evaluate its stability in relation to physical factors that can affect the quality and maintenance of product characteristics. To do this, different aliquots of FARC batch IP-TOT1/11 submitted to temperatures of 37, 56, 65 and 80°C for 1hour or exposed to ambient light for 10, 20, 30, 45, 60, 90, 120, 150 minutes and 24 hours were used on direct immunefluorescence (DIF)... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/3071 |
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| Troti, A. C. P.; Silva, A. C. R.; Peixoto, Z. M. P.; Ferreira, K. C. S.; Chaves, L. B.; Caporale, G. M. M.. |
| Stability is defined as the period during which a product or reactant has, within specified limits, the same properties and characteristics possessed at the time of its manufacture. The determination of the validity ensures that the reagent maintains the characteristics of strength, purity and quality during the proposed period when stored under appropriate conditions. It is recommended for maintenance of conjugated polyclonal or antiribonucleoproteins rabies virus, at 4°C and protection of light incidence. The objective of this study was to evaluate the stability of the title of lots of rabies virus fluorescent conjugate produced at the Pasteur Institute of São Paulo (IP/SP), adequately stored for different periods and temperatures. The titles of the... |
| Tipo: Info:eu-repo/semantics/article |
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| Ano: 2013 |
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/5393 |
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